Murine HIV p24 specific T lymphocyte activation by different antigen presenting cells: B lumphocytes from immunised mice present core protein to T-cells

Abstract

Mice were injected with three doses of baculovirus-produced recombinant HIV-1 p24 core protein in alum adjuvant. CD4 positive T lymphocytes from immunized animals proliferated in vitro in the presence of antigen and peritoneal macrophages (Mps) or splenic dendritic cells (DCs) from non-immunized mice as antigen presenting cells (APCs). DCs were approximately three times more efficient than Mps on a cell for cell basis. No synergy was observed between Mps and DCs in this system. B lymphocytes from immunized animals also presented p24 antigen to the specific T cells. Mps did synergize with B cells to enhance the level of T lymphocyte proliferation. This may have implications for the induction of specific immune responses to pathogens after administration of single protein vaccines.