Atrophy of non-locomotor muscle in patients with end-stage renal failure



Sakkas, G K and Sargeant, A J and Mercer, Tom and Ball, Derek and Tolfrey, K and Naif, PF (2003) Atrophy of non-locomotor muscle in patients with end-stage renal failure. Nephrology Dialysis Transplantation, 18 (10). pp. 2074-2081. ISSN 09310509

[img] PDF
eResearch_94.pdf
Restricted to Repository staff only

Download (294kB)

Abstract

Background. All previous histological studies of skeletal muscles of patients with renal failure have used locomotor muscle biopsies. It is thus unclear to what degree the observed abnormalities are due to the uraemic state and how much is due to disuse. The present study was undertaken to attempt to investigate this question by examining a non-locomotor muscle (rectus abdominis) in patients with end-stage renal failure. Methods. Biopsies from rectus abdominis were obtained from 22 renal failure patients (RFPs) undergoing surgical Tenchkoff catheter implantation for peritoneal dialysis and 20 control subjects undergoing elective abdominal surgery. Histochemical staining of frozen sections and morphometric analysis was used to estimate the proportion of each fibre type, muscle fibre area and capillary density. Myosin heavy chain composition was examined by SDS–PAGE. Results. There were no differences in fibre type distribution between RFPs and controls. All RFPs showed fibre atrophy [mean cross-sectional area (CSA) 3300 ± 1100 µm2, compared to 4100 ± 1100 µm2 in controls (P < 0.05)]. All fibre types were smaller in mean CSA in RFPs than in controls (15, 26 and 28% for types I, IIa and IIx, respectively). These differences could not be accounted for by differences in age, gender or cardiovascular or diabetic comorbidity. Muscle fibre capillarization, expressed as capillaries per fibre or capillary contacts per fibre, was significantly less in RFPs. Conclusions. Since a non-locomotor muscle was examined, the effects of disuse as a cause of atrophy have been minimized. It is likely, therefore, that the decreased muscle fibre CSA and capillary density of RFPs compared to controls were due predominantly to uraemia itself.

Item Type: Article
Additional Information: Research publication emanating from an externally funded (Greek Government Scholarship) PhD study (Sakkas; Sargeant as Director of Studies and Mercer as Supervisor) and activities of Renal Rehabilitation research group led by Mercer and Naish.
Uncontrolled Keywords: atrophy; non-locomotor muscle; renal failure
Divisions: School of Health Sciences > Physiotherapy
Date Deposited: 22 May 2008 11:03
Last Modified: 19 Mar 2014 12:54
URI: http://eresearch.qmu.ac.uk/id/eprint/94

Actions (login required)

View Item View Item