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dc.contributor.authorAnthony, J. P.
dc.contributor.authorFyfe, Lorna
dc.contributor.authorStewart, D.
dc.contributor.authorMcDougall, G. J.
dc.contributor.authorSmith, H. V.
dc.date.accessioned2018-06-29T21:33:10Z
dc.date.available2018-06-29T21:33:10Z
dc.date.issued2007-08
dc.identifierER2929
dc.identifier.citationAnthony, J., Fyfe, L., Stewart, D., McDougall, G. & Smith, H. (2007) The effect of blueberry extracts on Giardia duodenalis viability and spontaneous excystation of Cryptosporidium parvum oocysts, in vitro. Methods, 42(4), pp. 339-348.
dc.identifier.urihttps://doi.org/10.1016/j.ymeth.2007.02.011
dc.identifier.urihttps://eresearch.qmu.ac.uk/handle/20.500.12289/2929
dc.description.abstractThe protozoan parasites Giardia duodenalis and Cryptosporidium parvum are common causes of diarrhoea, worldwide. Effective drug treatment is available for G. duodenalis, but with anecdotal evidence of resistance or reduced compliance. There is no effective specific chemotherapeutic intervention for Cryptosporidium. Recently, there has been renewed interest in the antimicrobial properties of berries and their phenolic compounds but little work has been done on their antiparasitic actions. The effect of various preparations of blueberry (Vaccinium myrtillus) extract on G. duodenalis trophozoites and C. parvum oocysts were investigated. Pressed blueberry extract, a polyphenolic-rich blueberry extract, and a commercially produced blueberry drink (Bouvrage) all demonstrated antigiardial activity. The polyphenol-rich blueberry extract reduced trophozoite viability in a dose dependent manner. At 167 microgml(-1), this extract performed as well as all dilutions of pressed blueberry extract and the Bouvrage beverage (9.6+/-2.8% live trophozoites remaining after 24h incubation). The lowest dilution of blueberry extract tested (12.5% v/v) contained >167 microgml(-1) of polyphenolic compounds suggesting that polyphenols are responsible for the reduced survival of G. duodenalis trophozoites. The pressed blueberry extract, Bouvrage beverage and the polyphenolic-rich blueberry extract increased the spontaneous excystation of C. parvum oocysts at 37 degrees C, compared to controls, but only at a dilution of 50% Bouvrage beverage, equivalent to 213 microgml(-1) gallic acid equivalents in the polyphenolic-rich blueberry extract. Above this level, spontaneous excystation is decreased. We conclude that water soluble extracts of blueberries can kill G. duodenalis trophozoites and modify the morphology of G. duodenalis and C. parvum.
dc.format.extent339-348
dc.publisherElsevier
dc.relation.ispartofMethods
dc.titleThe effect of blueberry extracts on Giardia duodenalis viability and spontaneous excystation of Cryptosporidium parvum oocysts, in vitro
dc.typearticle
dcterms.accessRightsnone
dc.description.facultysch_die
dc.description.volume42
dc.identifier.doi10.1016/j.ymeth.2007.02.011
dc.description.ispublishedpub
dc.description.eprintid2929
rioxxterms.typearticle
qmu.authorFyfe, Lorna
dc.description.statuspub
dc.description.number4


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