dc.contributor.author | Travers, M. T. | |
dc.contributor.author | Gow, Iain F. | |
dc.contributor.author | Barber, M. C. | |
dc.contributor.author | Thomson, J. | |
dc.contributor.author | Shennan, D. B. | |
dc.date.accessioned | 2018-06-29T21:34:07Z | |
dc.date.available | 2018-06-29T21:34:07Z | |
dc.date.issued | 2004-02-10 | |
dc.identifier | ER4386 | |
dc.identifier.citation | Travers, M., Gow, I., Barber, M., Thomson, J. & Shennan, D. (2004) Indoleamine 2,3-dioxygenase activity and L-tryptophan transport in human breast cancer cells., Biochimica et biophysica acta, vol. 1661, , pp. 106-12, | |
dc.identifier.issn | Jun-02 | |
dc.identifier.uri | https://eresearch.qmu.ac.uk/handle/20.500.12289/4386 | |
dc.description.abstract | The activity and expression of indoleamine 2,3-dioxygenase together with L-tryptophan transport has been examined in cultured human breast cancer cells. MDA-MB-231 but not MCF-7 cells expressed mRNA for indoleamine 2,3-dioxygenase. Kynurenine production by MDA-MB-231 cells, which was taken as a measure of enzyme activity, was markedly stimulated by interferon-gamma (1000 units/ml). Accordingly, L-tryptophan utilization by MDA-MB-231 cells was enhanced by interferon-gamma. 1-Methyl-DL-tryptophan (1 mM) inhibited interferon-gamma induced kynurenine production by MBA-MB-231 cells. Kynurenine production by MCF-7 cells remained at basal levels when cultured in the presence of interferon-gamma. L-Tryptophan transport into MDA-MB-231 cells was via a Na(+)-independent, BCH-sensitive pathway. It appears that system L (LAT1/CD98) may be the only pathway for l-tryptophan transport into these cells. 1-Methyl-D,L-tryptophan trans-stimulated l-tryptophan efflux from MDA-MB-231 cells and thus appears to be a transported substrate of system L. The results suggest that system L plays an important role in providing indoleamine-2,3-dioxygenase with its main substrate, L-tryptophan, and suggest a mechanism by which estrogen receptor-negative breast cancer cells may evade the attention of the immune system. | |
dc.format.extent | 106-12 | |
dc.publisher | Science Direct | |
dc.relation.ispartof | Biochimica et biophysica acta | |
dc.title | Indoleamine 2,3-dioxygenase activity and L-tryptophan transport in human breast cancer cells. | |
dc.type | article | |
dcterms.accessRights | none | |
dc.description.faculty | sch_die | |
dc.description.volume | 1661 | |
dc.description.ispublished | pub | |
dc.description.eprintid | 4386 | |
rioxxterms.type | article | |
refterms.dateAccepted | 2004-02-10 | |
qmu.author | Gow, Iain F. | |
dc.description.status | pub | |
dc.description.number | 1 | |