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dc.contributor.authorTravers, M. T.
dc.contributor.authorGow, Iain F.
dc.contributor.authorBarber, M. C.
dc.contributor.authorThomson, J.
dc.contributor.authorShennan, D. B.
dc.date.accessioned2018-06-29T21:34:07Z
dc.date.available2018-06-29T21:34:07Z
dc.date.issued2004-02-10
dc.identifierER4386
dc.identifier.citationTravers, M., Gow, I., Barber, M., Thomson, J. & Shennan, D. (2004) Indoleamine 2,3-dioxygenase activity and L-tryptophan transport in human breast cancer cells., Biochimica et biophysica acta, vol. 1661, , pp. 106-12,
dc.identifier.issnJun-02
dc.identifier.urihttps://eresearch.qmu.ac.uk/handle/20.500.12289/4386
dc.description.abstractThe activity and expression of indoleamine 2,3-dioxygenase together with L-tryptophan transport has been examined in cultured human breast cancer cells. MDA-MB-231 but not MCF-7 cells expressed mRNA for indoleamine 2,3-dioxygenase. Kynurenine production by MDA-MB-231 cells, which was taken as a measure of enzyme activity, was markedly stimulated by interferon-gamma (1000 units/ml). Accordingly, L-tryptophan utilization by MDA-MB-231 cells was enhanced by interferon-gamma. 1-Methyl-DL-tryptophan (1 mM) inhibited interferon-gamma induced kynurenine production by MBA-MB-231 cells. Kynurenine production by MCF-7 cells remained at basal levels when cultured in the presence of interferon-gamma. L-Tryptophan transport into MDA-MB-231 cells was via a Na(+)-independent, BCH-sensitive pathway. It appears that system L (LAT1/CD98) may be the only pathway for l-tryptophan transport into these cells. 1-Methyl-D,L-tryptophan trans-stimulated l-tryptophan efflux from MDA-MB-231 cells and thus appears to be a transported substrate of system L. The results suggest that system L plays an important role in providing indoleamine-2,3-dioxygenase with its main substrate, L-tryptophan, and suggest a mechanism by which estrogen receptor-negative breast cancer cells may evade the attention of the immune system.
dc.format.extent106-12
dc.publisherScience Direct
dc.relation.ispartofBiochimica et biophysica acta
dc.titleIndoleamine 2,3-dioxygenase activity and L-tryptophan transport in human breast cancer cells.
dc.typearticle
dcterms.accessRightsnone
dc.description.facultysch_die
dc.description.volume1661
dc.description.ispublishedpub
dc.description.eprintid4386
rioxxterms.typearticle
refterms.dateAccepted2004-02-10
qmu.authorGow, Iain F.
dc.description.statuspub
dc.description.number1


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