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dc.contributor.authorMarenah, Lamin
dc.contributor.authorMcCluskey, Jane T.
dc.contributor.authorAbdel-Wahab, Yasser H.
dc.contributor.authorO'Harte, Finbarr P. M.
dc.contributor.authorMcClenaghan, Neville H.
dc.contributor.authorFlatt, Peter R.
dc.date.accessioned2018-06-29T21:34:04Z
dc.date.available2018-06-29T21:34:04Z
dc.date.issued2006-07
dc.identifierER4493
dc.identifier.citationMarenah, L., McCluskey, J., Abdel-Wahab, Y., O''Harte, F., McClenaghan, N. & Flatt, P. (2006) A stable analogue of glucose-dependent insulinotropic polypeptide, GIP(LysPAL16), enhances functional differentiation of mouse embryonic stem cells into cells expressing islet-specific genes and hormones, Biological Chemistry, vol. 387, , pp. 941-947,
dc.identifier.issn1431-6730
dc.identifier.urihttp://doi.org/10.1515/BC.2006.118
dc.identifier.urihttps://eresearch.qmu.ac.uk/handle/20.500.12289/4493
dc.description.abstractEmbryonic stem (ES) cells can be differentiated into insulin-producing cells by conditioning the culture media. However, the number of insulin-expressing cells and amount of insulin released is very low. Glucose-dependent insulinotropic polypeptide (GIP) enhances the growth and differentiation of pancreatic beta-cells. This study examined the potential of the stable analogue GIP(LysPAL16) to enhance the differentiation of mouse ES cells into insulin-producing cells using a five-stage culturing strategy. Semi-quantitative PCR indicated mRNA expression of islet development markers (nestin, Pdx1, Nkx6.1, Oct4), mature pancreatic beta-cell markers (insulin, glucagon, Glut2, Sur1, Kir6.1) and the GIP receptor gene GIP-R in undifferentiated (stage 1) cells, with increasing levels in differentiated stages 4 and 5. IAPP and somatostatin genes were only expressed in differentiated stages. Immunohistochemical studies confirmed the presence of insulin, glucagon, somatostatin and IAPP in differentiated ES cells. After supplementation with GIP(LysPAL16), ES cells at stage 4 released insulin in response to secretagogues and glucose in a concentration-dependent manner, with 35-100% increases in insulin release. Cellular C-peptide content also increased by 45% at stages 4 and 5. We conclude that the stable GIP analogue enhanced differentiation of mouse ES cells towards a phenotype expressing specific beta-cell genes and releasing insulin.
dc.format.extent941-947
dc.publisherDe Gruyter
dc.relation.ispartofBiological Chemistry
dc.titleA stable analogue of glucose-dependent insulinotropic polypeptide, GIP(LysPAL16), enhances functional differentiation of mouse embryonic stem cells into cells expressing islet-specific genes and hormones
dc.typearticle
dcterms.accessRightsnone
dc.description.facultysch_die
dc.description.volume387
dc.identifier.doihttp://doi:10.1515/BC.2006.118
dc.description.ispublishedpub
dc.description.eprintid4493
rioxxterms.typearticle
qmu.authorMcCluskey, Jane T.
dc.description.statuspub
dc.description.number7


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