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dc.contributor.authorHamid, M.
dc.contributor.authorMcCluskey, Jane T.
dc.contributor.authorMcClenaghan, N. H.
dc.contributor.authorFlatt, P. R.
dc.date.accessioned2018-06-29T21:32:53Z
dc.date.available2018-06-29T21:32:53Z
dc.date.issued2001-06
dc.identifierER4505
dc.identifier.citationHamid, M., McCluskey, J., McClenaghan, N. & Flatt, P. (2001) FUNCTIONAL EXAMINATION OF MICROENCAPSULATED BIOENGINEERED INSULIN-SECRETING BETA-CELLS, Cell Biology International, vol. 25, , pp. 553-556,
dc.identifier.issn10656995
dc.identifier.urihttp://doi.org/10.1006/cbir.2000.0663
dc.identifier.urihttps://eresearch.qmu.ac.uk/handle/20.500.12289/4505
dc.description.abstractClonal insulin-secreting BRIN-BD11 cells engineered by electrofusion were encapsulated inside natrium alginate beads and cultured in RPMI 1640 culture media. Acute insulin secretory responses to glucose and amino acids were compared between microencapsulated cells and non-encapsulated cells maintained in monolayer culture. Encapsulated cells exhibited a 1.5-fold, 2.9-fold and 4.2-fold increase (P< 0.001) in insulin release in response to 16.7 mmol/l glucose, 10 mmol/l L-arginine and 10 mmol/l L-alanine respectively. Insulin output by non-encapsulated cells was approximately 30% greater but the relative magnitudes of responses were similar. This is the first study to demonstrate the stability of cellular engineered insulin-secreting cells encapsulated in alginate beads, illustrating the utility of this approach for cellular engineering and potential transplantation in diabetes. Copyright 2001 Academic Press.
dc.format.extent553-556
dc.publisherWiley
dc.relation.ispartofCell Biology International
dc.titleFUNCTIONAL EXAMINATION OF MICROENCAPSULATED BIOENGINEERED INSULIN-SECRETING BETA-CELLS
dc.typearticle
dcterms.accessRightsnone
dc.description.facultysch_die
dc.description.volume25
dc.identifier.doihttp://doi:10.1006/cbir.2000.0663
dc.description.ispublishedpub
dc.description.eprintid4505
rioxxterms.typearticle
qmu.authorMcCluskey, Jane T.
dc.description.statuspub
dc.description.number6


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