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    Cyp11b1 Null Mouse, a Model of Congenital Adrenal Hyperplasia

    Date
    2008-11
    Author
    Mullins, L. J.
    Peter, A.
    Wrobel, N.
    McNeilly, J. R.
    McNeilly, A. S.
    Al-Dujaili, Emad A. S.
    Brownstein, D. G.
    Mullins, J. J.
    Kenyon, C. J.
    Metadata
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    Citation
    Mullins, L., Peter, A., Wrobel, N., McNeilly, J., McNeilly, A., Al-Dujaili, E., Brownstein, D., Mullins, J. & Kenyon, C. (2008) Cyp11b1 Null Mouse, a Model of Congenital Adrenal Hyperplasia, Journal of Biological Chemistry, vol. 284, , pp. 3925-3934,
    Abstract
    Patients with congenital adrenal hyperplasia arising from mutations of 11_-hydroxylase, the final enzyme in the glucocorticoid biosynthetic pathway, exhibit glucocorticoid deficiency, adrenal hyperplasia driven by unsuppressed hypothalamo-pituitary-adrenal activity, and excess mineralocorticoid activity caused by the accumulation of deoxycorticosterone. A mouse model, in which exons 3-7 of Cyp11b1 (the gene encoding 11_-hydroxylase) were replaced with cDNA encoding enhanced cyan fluorescent protein, was generated to investigate the underlying disease mechanisms. Enhanced cyan fluorescent protein was expressed appropriately in the zona fasciculata of the adrenal gland, and targeted knock-out was confirmed by urinary steroid profiles and, immunocytochemically, by the absence of 11_-hydroxylase. The null mice exhibited glucocorticoid deficiency, mineralocorticoid excess, adrenal hyperplasia, mild hypertension, and hypokalemia. They also displayed glucose intolerance. Because rodents do not synthesize adrenal androgens, changes in reproductive function such as genital virilization of females were not anticipated. However, adult homozygote females were infertile, their ovaries showing an absence of corpora lutea and a central proliferation of disorganized steroidogenic tissue. Null females responded normally to superovulation, suggesting that raised systemic progesterone levels also contribute to infertility problems. The model reveals previously unrecognized phenotypic subtleties of congenital adrenal hyperplasia.
    Official URL
    http://dx.doi.org/10.1074/jbc.M805081200
    URI
    https://eresearch.qmu.ac.uk/handle/20.500.12289/782
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    • Dietetics, Nutrition and Biological Sciences

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