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dc.contributor.authorSakkas, G. K.
dc.contributor.authorBall, Derek
dc.contributor.authorMercer, Tom
dc.contributor.authorNaish, P. F.
dc.date.accessioned2018-06-29T21:45:44Z
dc.date.available2018-06-29T21:45:44Z
dc.date.issued2003-06
dc.identifierER964
dc.identifier.citationSakkas, G., Ball, D., Mercer, T. & Naish, P. (2003) An alternative histochemical method to simultaneously demonstrate muscle nuclei and muscle fibre type, European Journal of Applied Physiology, vol. 89, , pp. 503-505,
dc.identifier.issn14396319
dc.identifier.urihttp://dx.doi.org/10.1007/s00421-003-0829-z
dc.identifier.urihttps://eresearch.qmu.ac.uk/handle/20.500.12289/964
dc.description.abstractWe present a modified histochemical method to examine, simultaneously, nuclei and fibre type in human skeletal muscle. The new procedure (Haem-ATPase) is based on two previously used histochemical protocols. Biopsies were obtained from the rectus abdominis muscle of patients undergoing elective abdominal surgery. Fibre type composition, cross-sectional area (CSA) and nuclei to fibre ratio (N:F) were determined from frozen sections of each biopsy. To test the validity of the new method, serial sections of each biopsy were stained separately using the standard and modified methods. No differences were found in fibre type distribution, mean-weighted CSA and N:F when comparing the modified method with the standard methods. The Haem-ATPase method was found to shrink fibre size by at least 3% (P>0.05) compared with the established myosin acid labile method. We propose that this modified technique is suitable for initial examination of both the nuclei and fibre type in the same frozen sections of human skeletal muscle.
dc.format.extent503-505
dc.publisherSpringer Verlag
dc.relation.ispartofEuropean Journal of Applied Physiology
dc.subjectMyosin ATPase Haematoxylin Skeletal muscle Fibre type Nuclei
dc.titleAn alternative histochemical method to simultaneously demonstrate muscle nuclei and muscle fibre type
dc.typearticle
dcterms.accessRightsrestricted
dc.description.facultysch_phy
dc.description.referencetextAllen DL, Roy RR, Edgerton VR (1999) Myonuclear domains in muscle adaptation and disease. Muscle Nerve 22:1350-1360 Bland JM, Altman DG (1986) Statistical methods for assessing agreement between two methods of clinical measurement. The Lancet 8:307-310 Brooke MH, Kaiser KK (1970) Muscle fibre types: how many and what kind? Arch Neurol (Chicago) 23:369-379 Guth L, Samaha FJ (1970) Procedure for the histochemical demonstration of actomyosin ATPase. Exp Neurol 28:365-367 Haggmark T, Thorstensson A (1979) Fibres types in human abdominal muscles. Acta Physiol Scand 107:319-325 Harris HF (1900) On rapid conversion of haematoxylin into haematein in staining reactions. J Appl Micro Lab Methods 3:777-778 Kadi F (2000) Adaptation of human skeletal muscle to training and anabolic steroids. Acta Physiol Scand [Suppl] 646:1-52 McCall GE, Allen DL, Linderman JK, Grindeland RE, Roy RR, Mukku VR, Edgerton VR (1998) Maintenance of myonuclear domain size in rat soleus after overload and growth hormone/ IGF-I treatment. J Appl Physiol 84:1407-1412
dc.description.volume89
dc.identifier.doihttp://10.1007/s00421-003-0829-z
dc.description.ispublishedpub
dc.description.eprintid964
rioxxterms.typearticle
qmu.authorMercer, Tom
dc.description.statuspub
dc.description.number5


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