Liu, H. K.Green, B. D.Gault, V. A.McCluskey, Jane T.McLenaghan, N. H.O'Harte, F. P. M.Flatt, P. R.2018-06-292018-06-292004Liu, H., Green, B., Gault, V., McCluskey, J., McLenaghan, N., O''Harte, F. & Flatt, P. (2004) N-acetyl-GLP-1: a DPP IV-resistant analogue of glucagon-like peptide-1 (GLP-1) with improved effects on pancreatic _-cell-associated gene expression, Cell Biology International, vol. 28, pp. 69-73.10656995http://doi.org/10.1016/j.cellbi.2003.10.004https://eresearch.qmu.ac.uk/handle/20.500.12289/4498Glucagon-like peptide-1(7-36)amide (GLP-1) is a key insulinotropic hormone with the reported potential to differentiate non-insulin secreting cells into insulin-secreting cells. The short biological half-life of GLP-1 after cleavage by dipeptidylpeptidase IV (DPP IV) to GLP-1(9-36)amide is a major therapeutic drawback. Several GLP-1 analogues have been developed with improved stability and insulinotropic action. In this study, the N-terminally modified GLP-1 analogue, N-acetyl-GLP-1, was shown to be completely resistant to DPP IV, unlike native GLP-1, which was rapidly degraded. Furthermore, culture of pancreatic ductal ARIP cells for 72 h with N-acetyl-GLP-1 indicated a greater ability to induce pancreatic beta-cell-associated gene expression, including insulin and glucokinase. Further investigation of the effects of stable GLP-1 analogues on beta-cell differentiation is required to assess their potential in diabetic therapy.69-73articlehttp://doi:10.1016/j.cellbi.2003.10.004