Barron, RonaStevenson, KarenInglis, Neil F.Klausen, JoanSharp, J. Michael2022-12-212022-12-211994-08-01Cameron, R.M., Stevenson, K., Inglis, N.F., Klausen, J. and Sharp, J.M. (1994) ‘Identification and characterization of a putative serine protease expressed in vivo by Mycobacterium avium subsp. paratuberculosis’, Microbiology, 140(8), pp. 1977–1982. Available at: https://doi.org/10.1099/13500872-140-8-1977.https://eresearch.qmu.ac.uk/handle/20.500.12289/12730https://doi.org/10.1099/13500872-140-8-1977Rona Barron - ORCID: 0000-0003-4512-9177 https://orcid.org/0000-0003-4512-9177Item not available from this repository.A putative serine protease expressed in vivo by Mycobacterium avium subsp. paratuberculosis was isolated from a lambda gt11 genomic expression library by screening with serum from a naturally infected sheep. The gene was contained in two overlapping clones, which were shown by antibody elution to encode a protein of 34 kDa in M. a. paratuberculosis. The clones were sequenced and database searches detected a motif identical to the active serine site in trypsin, and 30% homology to the putative serine proteases (HtrA proteins) of Escherichia coli, Salmonella typhimurium, Brucella abortus and Rochalimaea henselae.1977–82enArticle