CC BY-NC-ND 4.0 Attribution-NonCommercial-NoDerivatives 4.0 InternationalLonchamp, JulienEuston, Stephen R.2025-01-162025-01-162025-01-18Lonchamp, J. and Euston, S.R. (2025) ‘Extraction of an emulsifying agent from the cellulose-based filtration aid of cold-pressed rapeseed oil’, Food Bioscience, 64, p. 105930. Available at: https://doi.org/10.1016/j.fbio.2025.105930.https://eresearch.qmu.ac.uk/handle/20.500.12289/14113https://doi.org/10.1016/j.fbio.2025.105930Julien Lonchamp - ORCID: 0000-0001-7954-4745 https://orcid.org/0000-0001-7954-4745AAM updated with VoR 21/01/2025This study aimed to extract an emulsifying agent from the cellulose-based filtration aid of cold-pressed rapeseed oil. This material is currently a low-value stream but contains phospholipids retained during filtration and proteins from residual seed peel and pulp particles. A range of two-step extraction methods were assessed, with oil removal using diethyl ether (DE) or petroleum ether followed by chloroform-methanol (CM) extraction (2:1, 3:2 or 5:4 ratios) or the reversed order. Emulsions prepared with the DE/CM3:2 extract displayed the highest emulsifying activity and stability indexes (35.52 m2/g and 2,045.18 min) and similar small mean oil droplet sizes (3 μm) to those of whey protein concentrate emulsions. A range of extracted compounds contributed to the DE/CM3:2 functionality, including napins, cruciferins and phospholipids. This study demonstrated for the first time the potential to upcycle this co-product by recovering emulsifying agents. Future studies will focus on optimising the extraction process and yield.105930en© 2025 The Authors. Published by Elsevier Ltd.http://creativecommons.org/licenses/by-nc-nd/4.0/RapeseedCo-productExtractionEmulsifierProteinPhospholipidArticle