Direct binding procedure of proteins and enzymes to fine magnetic particles
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Date
2002-03-15
Citation
Koneracká, M., Kopčanský, P., Timko, M., Ramchand, C.N., de Sequeira, A. and Trevan, M. (2002) ‘Direct binding procedure of proteins and enzymes to fine magnetic particles’, Journal of Molecular Catalysis B: Enzymatic, 18(1-3), pp. 13-18.
Abstract
Several clinically important proteins and enzymes (bovine serum albumin (BSA), glucose oxidase (GOD) (EC 1.1.3.4), streptokinase (EC 3.4.99.0), chymotrypsin (EC 3.4.21.1) and dispase (EC 3.4.24.3)), respectively, have been immobilised onto fine magnetic particles using carbodiimide as a coupling agent. The coupling reactions of these substances were carried out using various ratios of magnetic particles to protein, and different values of pH to determine the optimum conditions of immobilisation. The possible applications in biomedicine and biotechnology of this method of immobilisation are discussed.