Dietetics, Nutrition and Biological Sciences
Permanent URI for this collectionhttps://eresearch.qmu.ac.uk/handle/20.500.12289/23
Browse
3 results
Search Results
Item The effect of green-coffee-bean extract rich in chlorogenic acid on antioxidant status of healthy human volunteers(Cambridge University Press, 2010-01) Almoosawi, Suzana; Tsang, Catherine; Davidson, Isobel M; Fyfe, LornaSeveral studies have linked consumption of green-coffee-bean extract (GCBE) rich in chlorogenic acid (CGA) with reduced blood pressure(Reference Watanabe, Arai and Mitsui1–Reference Ochiai, Jokura and Suzuki3). It is hypothesised that increased antioxidant activity could be one of the underlying mechanisms by which GCBE reduces blood pressure. To test this hypothesis a GCBE preparation rich in CGA was assessed by three extraction methods for antioxidant activity. In vivo antioxidant activity was also determined in a group of healthy volunteers. The phenolic content of GCBE, as determined by the Folin-Ciocalteu method, varied greatly between the ethanolic acidified water (0.2% (v/v) formic acid) and acetonitrile extract (acetonitrile–water containing 0.2% (v/v) formic acid; 50:50, v/v): 123 (sd 0.23), 131 (sd 0.66) and 211 (sd 0.51) mg gallic acid equivalents (GAE)/g extract respectively. Similar findings were observed with the Fe3+-reducing ability of plasma (FRAP) assay, wherein the acetonitrile extract exhibited a stronger Fe3+-reducing ability than the ethanolic extract (0.067 mmol/g extract v. 0.048 mmol/g extract). The 2,2-di(4-tert-octylphenyl)-1-picrylhydrazyl (DPPH) radical-scavenging activity of the ethanolic extract of GCBE was 70.4% at 50 μm as compared with ascorbic acid (86.1% inhibition at 50 μm) and GAE (82.2% at 50 μm). To examine in vivo antioxidant properties of GCBE thirteen healthy volunteers (age 36 (sd 11) years, BMI 28 (sd 2.5) kg/m2) consumed 200 mg GCBE containing 90 mg CGA twice daily for 2 weeks. In vivo antioxidant activity was determined using the Folin-Ciocalteu method and FRAP. There was a significant correlation between urinary polyphenols excretion as determined by the Folin-Ciocalteu method and FRAP (0.664, P<0.0001). However no significant increase in urinary antioxidant activity was observed (total phenolics: 173.2 (sd 137.8) mg GAE/g creatinine v. 175.20 (sd 115.7) mg GAE/g creatinine, P>0.05; FRAP: 2.07 (sd 0.9) mmol Fe2+/g creatinine v. 1.56 (sd 0.7) mmol Fe2+/g creatinine, P>0.05). Systolic blood pressure decreased from 119 (sd 10.5) to 114 (sd 9.1) mmHg (P=0.05) following the 2-week treatment. In conclusion, green coffee bean extract has a high antioxidant activity. However, no changes in antioxidant activity are observed in urine. This finding is consistent with previous findings of poor antioxidant activity of hippuric acid, the main urinary metabolite of chlorogenic acid(Reference Olthof, Hollman and Buijsman4). Further research is required to identify the mechanism(s) of reduction in blood pressure. The antioxidant activity of plasma should also be determined.Item Effects of green tea consumption on blood pressure, total cholesterol, body weight and fat in healthy volunteers(2009) Al-Dujaili, Emad A. S.; Bradley, Jon-Paul; Almoosawi, Suzana; Fyfe, LornaBackground: Hypertension, obesity and hyperlipidemia are key interlinked features of both metabolic syndrome and cardiovascular disease. Numerous studies have suggested that green tea may reduce blood pressure by activating endothelial nitric oxide synthase and reducing total cholesterol by disrupting the production of apo B and synthesis of chylomicrons and thus have cardio-protective effects. The aim of this pilot study was to investigate the effects of increasing the consumption of green tea-rich catechins on blood pressure (BP), total cholesterol and other body composition parameters in healthy volunteers living in Scotland. Methods: Following a 2 day green tea free period, participants (n=12; 9 females and 3 males) were asked to drink 4 cups of green tea (organic Mao Jian Green Tea from the Zhejiang region of China) for 14 days (~800 ml green tea infusion containing 600-800 mg total catechins). Fasting total plasma cholesterol, BP, weight, BMI and %body fat were measured at day 0 (baseline), day 7 and day 14. Results: Mean systolic BP was reduced significantly by 7.1 mmHg (P<0.0001) and mean diastolic BP reduced by 7.8 mmHg over 14 days (P<0.0001). Mean fasting total cholesterol was reduced significantly by 0.556 mmol/l (P<0.008), BMI by 0.34 kg/m2 (P<0.001), body weight by 0.96 kg (P<0.001) and body fat by 2.36% (P<0.005). Conclusion: Our results has shown that short term consumption of commercial green tea reduces systolic and diastolic BP, fasting total cholesterol, %body fat and body weight suggesting a role for green tea in decreasing established potential cardiovascular risk factors. This study also suggests that reductions may be more pronounced in the overweight population where a significant proportion are obese and have a high risk of cardiovascular disease. Green tea consumption is cost effective, accepted by patients and has no reported side effects.Item Effect of green coffee bean extract and chlorogenic acid consumption on 11_HSD activity in humans and mice(2009-03) Almoosawi, Suzana; Dickinson, A.; Fyfe, Lorna; Kenyon, C. J.; Al-Dujaili, Emad A. S.Increased 11_ hydroxysteroid dehydrogenase type 1 (11_HSD1) activity is implicated in the development of the metabolic syndrome. Identifying natural compounds that influence 11_HSD1 activity could lead to novel methods of treating obesity, cardiovascular disease and diabetes. In the present study, we tested the effect of green coffee bean extract (GCBE), rich in chlorogenic acid (CGA), in human volunteers and of CGA in mice on blood pressure (BP), lipid and glucose metabolism. Our hypothesis was that CGA would improve these parameters, by blocking the uptake of microsomal glucose-6-phosphate which in turn would limit the production of co-factor for 11_HSD1 reductase activity. With local Ethics Committee approval, 13 healthy overweight subjects were given GCBE containing 90 mg CGA twice daily for 2 weeks. Urinary 24 h free cortisol was reduced from 1.05230.45 to 0.7630.40 nmol/kg (P=0.07). Free cortisone excretion was reduced from 0.7120.38 to 0.4320.24 nmol/kg (P=0.007). Systolic BP decreased from 119.410.5 to 113.89.1 mmHg (P=0.05). Fasting plasma glucose (P=0.101), diastolic BP (P=0.114), free cortisol:cortisone ratio (P=0.216) and anthropometrical measurement were not affected. In vitro, 11_HSD1 activity (conversion of added cortisone to cortisol) in isolated mouse microsomes was inhibited dose-dependently by CGA. The effects of feeding diet containing 0.15% CGA for 17 days was tested in male C57BL6 mice. Adiposity was unaffected but liver (27.74.9 vs 15.52.2 mg/g, P<0.04) and plasma (1.240.18 vs 0.860.08 mg/ml, P<0.08) triglycerides tended to be reduced. Urinary 24 h cortisol excretion following IP injection of 20 mg/kg cortisone was 30.14.1 vs 24.25.3 nmol/kg (P<0.4) for control and CGA-treated mice. Peak plasma glucose levels in tolerance tests were earlier with CGA treatment although, over a 2 h period, glucose clearance was not affected.