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Dietetics, Nutrition and Biological Sciences

Permanent URI for this collectionhttps://eresearch.qmu.ac.uk/handle/20.500.12289/23

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Author: search.filters.author.Duncan, AndrewSubject: search.filters.subject.Critical Illness

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    Relation between pyridoxal and pyridoxal phosphate concentrations in plasma, red cells, and white cells in patients with critical illness
    (Oxford University Press, 2008-07-01) Vasilaki, Katerina; McMillan, Donald C.; Kinsella, John; Duncan, Andrew; O'Reilly, Denis St J.; Talwar, Dinesh
    Background: Evidence suggests that the relation between plasma and red cell vitamin B-6 concentrations is perturbed as part of the systemic inflammatory response in critically ill patients. Objective: The aim was to examine the cross-sectional and longitudinal interrelations between pyridoxal (PL) and pyridoxal phosphate (PLP) concentrations in plasma and red and white cells in patients with critical illness. Design: PLP and PL concentrations were measured by HPLC in plasma and red and white cells in normal subjects (n 126) and critically ill patients (n 96) on admission and on follow-up. Results: On admission, compared with the controls, median plasma PLP and PL (P 0.001 and 0.01, respectively) and red cell PLP and PL (P0.001 and0.05, respectively) andtheir ratio (PLP:PL) in plasma and red cells (P 0.001 and 0.01, respectively) were significantly lower in the critically ill. In critically ill patients, plasma PLP:PL was significantly lower than red cell PLP:PL (P 0.001) and white cell PLP:PL (P 0.008). Plasma PL concentration was directly associated with both red cell PL (rs 0.73, P 0.001) and white cell PL (rs 0.68, P 0.001). Red cell PL and white cell PL were directly associated with red cell PLP (rs 0.82, P 0.001) and white cell PLP (rs 0.68, P 0.001), respectively. Longitudinal measurements (n 48) were similar. Conclusions: The relation between plasma PLP and PL was significantly perturbed in critical illness. This effect was less pronounced in red and white cells. Therefore, these results confirm the hypothesis that intracellular PLP concentrations are more likely to be a reliable measure of status than are plasma measurements in the critically ill patient.
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    Relation between riboflavin, flavin mononucleotide and flavin adenine dinucleotide concentrations in plasma and red cells in patients with critical illness
    (Elsevier, 2010-07-24) Vasilaki, Katerina; McMillan, Donald C.; Kinsella, John; Duncan, Andrew; O'Reilly, Denis St J.; Talwar, Dinesh
    Background: There is some evidence that the relationship between plasma and red cell vitamin B2 concentrations is perturbed in the critically ill patient. The aim of the present study was to examine the longitudinal interrelationships between riboflavin, flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD) in plasma and red cells in patients with critical illness. Methods: Riboflavin, FMN and FAD concentrations were measured, by HPLC, in plasma and red cells in healthy subjects (n = 119) and in critically ill patients (n = 125) on admission and on follow-up. Results: On admission, compared with the controls, critically ill patients had significantly higher plasma riboflavin and FMN concentrations (p b 0.001) and lower median plasma FAD concentrations (p b 0.001). In the red cell, FAD concentrations were significantly lower in critically ill patients (p b 0.001). In healthy subjects, plasma riboflavin was directly associated with both plasma FMN (rs = 0.55, p b 0.001) and plasma FAD (rs = 0.49, p b 0.001). Red cell riboflavin was directly associated with red cell FMN (rs = 0.52, p b 0.001) but not red cell FAD. In the critically ill patients, plasma riboflavin was not significantly associated with either plasma FMN or FAD. Red cell riboflavin was directly associated with red cell FMN (rs = 0.79, p b 0.001) and red cell FAD (rs = 0.72, p b 0.001). Longitudinal measurements (n = 60) were similar. Conclusions: The relationship between plasma riboflavin, FMN and FAD was significantly perturbed in critical illness. This effect was less pronounced in red cells. Therefore, red cell FAD concentrations are more likely to be a reliable measure of status in the critically ill patient.