Identification and characterization of a putative serine protease expressed in vivo by Mycobacterium avium subsp. paratuberculosis
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Date
1994-08-01
Citation
Cameron, R.M., Stevenson, K., Inglis, N.F., Klausen, J. and Sharp, J.M. (1994) ‘Identification and characterization of a putative serine protease expressed in vivo by Mycobacterium avium subsp. paratuberculosis’, Microbiology, 140(8), pp. 1977–1982. Available at: https://doi.org/10.1099/13500872-140-8-1977.
Abstract
A putative serine protease expressed in vivo by Mycobacterium avium subsp. paratuberculosis was isolated from a lambda gt11 genomic expression library by screening with serum from a naturally infected sheep. The gene was contained in two overlapping clones, which were shown by antibody elution to encode a protein of 34 kDa in M. a. paratuberculosis. The clones were sequenced and database searches detected a motif identical to the active serine site in trypsin, and 30% homology to the putative serine proteases (HtrA proteins) of Escherichia coli, Salmonella typhimurium, Brucella abortus and Rochalimaea henselae.