Identification and characterization of a putative serine protease expressed in vivo by Mycobacterium avium subsp. paratuberculosis
Date
1994-08-01Author
Barron, Rona
Stevenson, Karen
Inglis, Neil F.
Klausen, Joan
Sharp, J. Michael
Metadata
Show full item recordCitation
Cameron, R.M., Stevenson, K., Inglis, N.F., Klausen, J. and Sharp, J.M. (1994) ‘Identification and characterization of a putative serine protease expressed in vivo by Mycobacterium avium subsp. paratuberculosis’, Microbiology, 140(8), pp. 1977–1982. Available at: https://doi.org/10.1099/13500872-140-8-1977.
Abstract
A putative serine protease expressed in vivo by Mycobacterium avium subsp. paratuberculosis was isolated from a lambda gt11 genomic expression library by screening with serum from a naturally infected sheep. The gene was contained in two overlapping clones, which were shown by antibody elution to encode a protein of 34 kDa in M. a. paratuberculosis. The clones were sequenced and database searches detected a motif identical to the active serine site in trypsin, and 30% homology to the putative serine proteases (HtrA proteins) of Escherichia coli, Salmonella typhimurium, Brucella abortus and Rochalimaea henselae.