Long-term beneficial effects of vanadate, tungstate, and molybdate on insulin secretion and function of cultured beta cells.
Green, Brian D.
McClenaghan, Neville H.
McCluskey, Jane T.
Flatt, Peter R.
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Liu, H., Green, B., McClenaghan, N., McCluskey, J. & Flatt, P. (2004) Long-term beneficial effects of vanadate, tungstate, and molybdate on insulin secretion and function of cultured beta cells., Pancreas, vol. 28, , pp. 364-8,
The ultratrace elements vanadate, tungstate, and molybdate exhibit significant antihyperglycemic effects in both type 1 and 2 diabetic animals, but possible effects on the function of pancreatic beta cells are understudied. In the present study, clonal BRIN BD11 cells were cultured for 3 days with each ultratrace element to establish doses lacking detrimental effects on viable beta cell mass. Vanadate treatment (4 micromol/L) had no effect on cellular insulin content but improved glucose-induced insulin secretory responsiveness. However, insulin secretion mediated by PKA and PKC activation was desensitized in vanadate-treated cells. Culture with tungstate (300 micromol/L) and molybdate (1 mmol/L) increased cellular insulin content and enhanced basal insulin release and the responsiveness to glucose and a wide range of other secretagogues. These observations suggest significant effects of ultratrace elements on pancreatic beta cells that may contribute to their antihyperglycemic action.