Oxidative stress-induced effects on pattern and pattern formation in cortical B50 neuronal cells in culture

Abstract

Oxidative stress adversely affects cells and tissues, and neuronal cells in particular have been shown to be more susceptible to the injurious effects of oxidative stress in which the cells may die when oxygen supply is reduced or completely eliminated. The aim of the present study was to study the effect of oxidative stress using hypoxia as a bench mark on the morphology of B50 neuronal cell lines cultured in hypoxia using neuronal pattern and pattern formation as case study. The B50 cells were cultured in normal incubator (21%O2; 5% CO2) as control group and hypoxic incubator (5%O2; 5% CO2) as the experimental group. Neuronal morphology, pattern and wellbeing were assessed using same field morphological assessment of cells and lactate dehydrogenase leakage (LDH). The result showed groups of dead and degenerating B50 neuronal cells, altered neuronal pattern and pattern formation and some significant changes (P<0.05) in cellular levels of LDH leakage in normal B50 cells and hypoxic cells. The changes in morphology, neuronal pattern and LDH release indicate that oxidative stress has induced morphological and cellular changes in cortical B50 cells in culture and that the B50 neuronal cells are susceptible to damage and injurious effects of oxidative stress represented by hypoxia as most brain cells.