Dietetics, Nutrition and Biological Sciences
Permanent URI for this collectionhttps://eresearch.qmu.ac.uk/handle/20.500.12289/23
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Item Un indicateur temps-temperature a phénol oxydase pour contrôler la nourriture refrigeree(1997-06-19) de Sequeira, Anil; Kelly, N. A.; Trevan, M.; Reuben, B. G.; Reuben, C. A.Item Development of enzymatic time-temperature indicators for stored foods [Oral Presentation](Campden and Chorleywood Food Research Association, 1999-05-25) de Sequeira, AnilItem A phenol oxidase time-temperature indicator for frozen/chilled food monitoring [Oral Presentation](Society of Chemical Industry, 2002-06-20) de Sequeira, Anil; Reuben, B. G.; Trevan, M.Item Direct binding procedure of proteins and enzymes to fine magnetic particles(Elsevier, 2002-03-15) Koneracká, M.; Kopčanský, P.; Timko, M.; Ramchand, C. N.; de Sequeira, Anil; Trevan, M.Several clinically important proteins and enzymes (bovine serum albumin (BSA), glucose oxidase (GOD) (EC 1.1.3.4), streptokinase (EC 3.4.99.0), chymotrypsin (EC 3.4.21.1) and dispase (EC 3.4.24.3)), respectively, have been immobilised onto fine magnetic particles using carbodiimide as a coupling agent. The coupling reactions of these substances were carried out using various ratios of magnetic particles to protein, and different values of pH to determine the optimum conditions of immobilisation. The possible applications in biomedicine and biotechnology of this method of immobilisation are discussed.Item Development of a time-temperature indicator based on immobilised banana phenol oxidase(PIRA International, 2004) de Sequeira, Anil; Ali, S.; Trevan, M.; Reuben, B.; Ver-Bruggen, SaraItem Time-temperature indicators [Oral Presentation](2004-04-07) de Sequeira, AnilItem Responsibilities of being a food scientist [Oral Presentation](Bath Spa University, 2005) de Sequeira, AnilItem Immobilization of enzymes on magnetic particles(Humana Press, 2006) Koneracká, Martina; Kopčanský, Peter; Timko, Milan; Ramchand, Chenyl Nynitapal; Saiyed, Zainul M.; Trevan, Michael; de Sequeira, Anil; Guisan, Jose M.Magnetic particles have been increasingly used as carriers for binding proteins, enzymes, and drugs. Such immobilization procedures for proteins, enzymes, antibodies, and other biologically active compounds have a major impact in different areas of biomedicine and biotechnology. The immobilized biomolecules can be used directly for a bioassay or as affinity ligands to capture or modify target molecules or cells. This chapter details immobilization procedures for proteins and enzymes onto various magnetically responsive carriers such as naked magnetic particles, carboxyl-modified microspheres, and aminomodified microspheres using direct binding procedure in the presence of coupling agents such as carbodiimide. The physical and chemical properties of freshly prepared magnetic particles were determined by magnetic measurements (VSM magnetometer), transmission electron microscopy (TEM), and atomic force microscopy (AFM). The extent of immobilization and enzyme activities were spectrophotometrically measured in order to find the retained activity after immobilization onto magnetic particles. The binding of proteins and enzymes was also confirmed by TEM microscopy.Item E for additives? [Oral Presentation](Bath Spa University, 2006) de Sequeira, AnilItem Responsibility to learn more about our food [Oral Presentation](University of East Anglia, 2006-09-04) de Sequeira, Anil
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