eResearch
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Item Comparison of the distribution and nervous innervation of the sensilla on the labrum of Gryllus bimaculatus (De Geer) and Acheta domesticus (L.) (Orthoptera : Gryllidae), and an account of their development in A. domesticus(Elsevier, 1984) Carline, Tom; Kubra, K.; Brown, V.; Beck, R.The labra of Gryllus bimaculatus (De Geer) and Acheta domesticus (L.) (Orthoptera : Gryllidae) were studied to reveal the distribution and nervous innervation of the sensilla, in order to provide morphological evidence of their function. Employing scanning electron microscopy and light microscopy, 9 types of sensilla are reported in G. bimaculatus and 10 types in A. domesticus. Of these sensilla, types 1 - 4 have setae ranging in size from 5 - 420 _m in G. bimaculatus and from 3 - 470 _m in A. domesticus. Secretory pores, coeloconic pegs, basiconic pegs and campaniform sensilla have been recorded in both species, while sensilla ampullacea are only present in A. domesticus. The different types of sensilla are found in discrete groups on the posterior surface of the labrum, but they are more randomly distributed on the anterior surface. The detailed innervation of the sensilla was revealed by means of nickel chloride infusion, followed by intensification using Timm's sulphide - silver technique. The labra of both species are innervated by 2 major branches of the labral nerve. Each sensillum is usually innervated by a bipolar neurone, although some sensilla have been shown to be innervated by multiterminal neurones. The dimensions and shapes of the cell bodies and dendrites are provided and differences between the 2 species are identified. In general, the cell bodies and dendrites are larger in G. bimaculatus than they are in A. domesticus. The development of the sensilla through the nymphal instars to the adult is reported for A. domesticus. It is shown that for some types of sensilla, (types 8 and 13), the number remains constant throughout the developmental period, in some (type 1), the adult complement of sensilla is attained in the early instars, for others, (types 2, 6, 7, 9 and 10), there is a steady increase throughout development, while in others (types 3 and 4), sudden increases occur at specific moults.Item Aeromonas salmonicida extracellular virulence factors: The common components [Project Report](International Council for the Exploration of the Sea. Maritime Committee, 1986) Coleman, G.; Fyfe, LornaItem A comparison of the distribution of extracellular proteins produced by the protease-secreting organism Aeromonas salmonicida during aerobic and anaerobic growth(1986) Fyfe, Lorna; Coleman, G.; Munro, A. L.Aeromonas salmonicida was grown aerobically and anaerobically in supplemented 3% (w/v) tryptone soya broth medium for 24 h at 25 degrees C. Although the bacterial density achieved was 4.9 times higher in the aerobic culture, the exoprotein produced per unit of bacterial dry weight was only 1.9 times higher than in the anaerobic culture. However, the protease activity of the exoprotein showed a marked reduction anaerobically, being only one-tenth of that of the exoprotein produced aerobically. This finding was consistent with the differing SDS-PAGE patterns of the extracellular proteins from the two cultures, which also showed marked loss and reinforcement of other, as yet unidentified extracellular products.Item A study of the pathological effect of isolated Aeromonas salmonicida extracellular protease on Atlantic salmon, Salmo salar(Wiley, 1986-09) Fyfe, Lorna; Finley, A.; Coleman, G.; Munro, A. L. S.A comparison was made between the effects of Aeromonas salmonidda extracellular protease and total extracellular products (ECP) following intramuscular injection into juvenile Atlantic salmon, Salmo salar L. Thus, 20, 10, 5, 2.5 and 1.5 units of salt-free protease in 0.2 ml water were compared with ECP preparations with the same levels of proteolytic activity. The highest concentration of ECP produced a gross pathology with a large furuncular lesion 36 h after injection. The corresponding protease preparation had a lesser effect, although a furuncle was formed and tissue liquefaction was produced. These effects were less marked with reduced concentrations. At the lowest level studied, no significant effect was observed with protease alone but ECP (0.8 _g of protein) produced a small, characteristic lesion similar to that achieved with 5 units of isolated protease.Item Nutrition, exercise and health: food for action, food for thought.(Ling Press, 1987) Mercer, Tom; Biddle, S. J. H.; Biddle, S. J. H.Item The electronic school library(1987) Harring, James E.In the vast literature now available on automation in libraries and information services of different kinds, relatively little has been written on the effects of automation, in particular the use of microcomputers, in school libraries. Yet it is in this area that some of the most interesting developments have taken place. It should also be remembered that the creation of the electronic school library brings information technology to all pupils in a school whereas in other libraries, e.g. public libraries, only the minority of the public who use the public library have access to the technology. The growing use of information technology linked to the development of information skills teaching in schools has, in Britain and elsewhere, created new roles for the school librarian and has enabled pupils to use information technology to think about and effectively use information as well as technology.Item A comparison of the characteristics of extracellular protein secretion by a Gram positive and a Gram negative bacterium(Plenum Press, 1987) Coleman, G.; Abbas, A.; Sutherland, J.; Fyfe, Lorna; Finley, A.; Chaloipka, J.; Krumphanzi, V.Item Incremental and constant load determinations of VO2 max and maximal constant-load performance time(1987) Lavoie, N. F.; Mercer, TomItem Some properties of a 56 kilodalton haemolysin specific for fish erythrocytes in the supernatant fraction of cultures of Aeromonas salmonicida [Project Report](International Council for Exploration of the Sea. Maritime Committee, 1987) Fyfe, LornaItem A comparative study of the formation of extracellular proteins by Aeromonas salmonicida at two different temperatures(Wiley, 1987-04) Fyfe, Lorna; Coleman, G.; Munro, A. L.Aeromonas salmonicida was grown in a supplemented 3% (w/v) tryptone soya broth medium at 10 degrees C, a temperature at the lower end of the range over which furunculosis has been observed to occur in the field, and 25 degrees C, the optimum temperature for growth. Similar bacterial densities in the range 2.35 +/- 0.05 mg dry wt/ml were achieved in the two cultures at the beginning of the stationary phase of the growth cycle, after 125 h at 10 degrees C and 18 h at 25 degrees C. At this point, at the higher temperature 1.5 times more exoprotein was formed, 80 +/- 2.8 micrograms/ml compared with 54 +/- 1.7 micrograms/ml. Exoprotein contained the same proportion of haemolysin at both temperatures and twice as much protease at the higher temperature. The most marked difference was in an unidentified 100 kD protein which was formed in a 10-fold greater amount at 10 degrees C.Item Identification of major common extracellular proteins secreted by Aeromonas salmonicida strains isolated from diseased fish(1987-04-01) Fyfe, Lorna; Coleman, G.; Munro, A. L.Ten different strains of Aeromonas salmonicida that were isolated from diseased fish were grown under identical conditions (24 h at 25 degree C) in 3% (wt/vol) tryptone soya broth medium supplemented with vitamins and inorganic ions. In each case the extracellular proteins that were formed were compared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and it was found that there were two significant common components, one with a molecular weight of 70,000 and the other with a weight of 56,000. Application of enzyme purification techniques to the supernatant fraction proteins of a culture of one of the strains resulted in the isolation of a 70-kilodalton (kDa) component, which was found to be a serine protease, and a 56-kDa component, which was hemolytic to trout erythrocytes. Rocket immunoelectrophoresis with rabbit antibodies to the isolated protease and hemolysin showed the same antigenic components in the supernatant fractions of all the cultures. These activities were assayed, and protease activity was found to vary by a factor of three, from 59 to 195 U/ml, while the range of hemolytic activity was over a narrow band, from 28 to 43 U/ml. There was an inconsistency between the immunoelectrophoretic and direct assay data in only one case. This indicated the presence of additional hemolytic activity, in addition to the 56-kDa component. The detection of large amounts of the same protease and hemolysin, two potent degradative activities, in a random series of strains of A. salmonicida suggests that they may be obligatory virulence factors in the development of furunculosis.Item Assessment of VO2 max of competitive cyclists using conventional ergometry versus a sports-specific test.(Illinois Human Kinetics, 1988) Lavoie, N. F.; Mercer, Tom; Ciolfi, M. A.; Burke, E R; Newsom, M. M.Item The combined effect of isolated Aeromanas salmonicida protease and haemolysin on Atlantic salmon, Salmo salar L. compared with that of total extra-cellular products preparation(Wiley, 1988-01) Fyfe, Lorna; Coleman, G.; Munro, A. L. S.Item The effectiveness of the three-year chiropody course in preparing for professional life(Routledge Taylor & Francis Group, 1988-01) Dunlop, G.Item Initial treatment of burns(Bailliere Tindall, 1989-08) Coull, AlisonItem Free tissue transfer(British Association of Plastic Surgery Nurses, 1990-03-31) Coull, Alison; Wylie, K.Item Regular monitoring: The way to ensure flap healing. Nursing priorities following flap repair and reconstruction surgery(Austen Cornish, 1990-10) Coull, Alison; Wylie, K.Surgical flap techniques are no longer confined to specialist plastic surgery units, and more nurses are being asked to manage patients following reconstructive surgery. Nursing management involves ensuring the flap's survival and healing.